PSV Eindhoven climbed to the top of the Eredivisie with a 4-2 victory over De Graafschap on Saturday.
Ajax extended their gap at the top of the Eredivisie with a narrow 1-0 victory at home to Vitesse on Saturday.
Michiel Kramer scored a hat-trick as Feyenoord won the Rotterdam derby 4-2 at Excelsior to move level with leaders Ajax,
Eredivisie leaders Ajax hammered Roda JC 6-0 to open up a three-point gap at the top of the table.
PSV Eindhoven and Heracles maintained their pressure on the Eredivisie's top two with late away wins on Saturday.
Feyenoord claimed second prize on the final day of the season as they booked a place in the Champions League, while Excelsior went down.
Ajax secured their 31st Dutch league title by beating VVV-Venlo in their penultimate game of the season.
Heerenveen are still in the fierce battle for a top-two finish in the Eredivisie, while Excelsior suffered a demoralising defeat.
Excelsior will be looking for a new coach in the summer after the board of directors decided not to continue with John Lammers in charge.
Twente kept the pressure on leaders Ajax after substitute Nacer Chadli grabbed a late winner at Excelsior Rotterdam on Saturday.
PSV Eindhoven secured a last-gasp success over AZ Alkmaar, but FC Twente conceded a late equaliser to put a dent in their hopes.
NEC Nijmegen moved into the Europa League play-off places after a 2-0 Eredivisie win at struggling Excelsior.
Young striker Mitchell te Vrede has joined Feyenoord on a free transfer from Excelsior, the Eredivisie giants have confirmed.
Tommy Oar scored a late winner as FC Utrecht grabbed a vital 3-2 win over Excelsior as they look to inch away from the relegation zone.
Excelsior remain in deep trouble towards the foot of the Eredivisie table after going down 1-0 at home to FC Groningen.
RKC Waalwijk enjoyed success against De Graafschap to pour more woe on the relegation candidates, while Excelsior earned a welcome win.
Teenage defender Jordy van Deelen has agreed a two-year deal at Feyenoord with the option for an extra year.
Heerenveen kept themselves firmly in the Eredivisie title hunt as ADO Den Haag boosted their top-flight survival hopes.
Excelsior have brought Luigi Bruins back to the club on a short-term deal until the end of the season in a bid to boost their survival hopes.
AZ Alkmaar took over at the top of the table, VVV Venlo and RKC claimed victories and Den Haag picked up an important point.