Serie A champions Juventus saw their 47-match unbeaten home league record come to an end against Udinese.
Cagliari drew a line under 11 years in Serie A by beating Udinese 4-3 in their last game before submitting to relegation.
Fiorentina qualified for the Europa League while Stephan El Shaarawy scored his first goal in seven months for AC Milan.
Liverpool give U21s more game time than Premier League rivals but top flight lags behind Europe.
A last-gasp winner from substitute Marco Sau lifted Cagliari to a 1-0 victory at Cesena.
Cagliari lost 1-0 at home to Palermo to suffer relegation and have their 11-year run in Serie A come to a painful end.
Paul Pogba made a goalscoring return for Juventus, but the Bianconeri had to settle for a 1-1 draw against Cagliari.
Paul Pogba made a goalscoring return for Juventus while AC Milan won their first match for over a month on Saturday.
Cagliari took full advantage of basement boys Parma's plight by plundering a vital 4-0 victory
Chievo beat relegation-threatened Cagliari on Wednesday evening to ensure themselves of Serie A football next season.
Juventus will have to wait another week to secure their status as Serie A champions after falling to a 2-1 defeat at Torino.
Duje Cop ensured Gianluca Festa's debut on the Cagliari bench went swimmingly with a 3-1 win at Fiorentina.
Italian Serie A side Cagliari have appointed former Middlesbrough defender Gianluca Festa as their new head coach
Zdenak Zeman has quit as coach of Cagliari just weeks into his second stint in charge of the Serie A strugglers.
Neither side could find the net in the Milan derby, while Roma missed the chance to go second.
Napoli are within five points of Roma and a Champions League place after a comfortable 3-0 Serie A victory at Cagliari.
Genoa left Cagliari even deeper in relegation danger with a 2-0 win at the Stadio Luigi Ferraris on Saturday.
Lazio kept the heat on second-place Roma by clinching a seventh consecutive Serie A success, 3-1 at 10-man Cagliari.
Juventus closed in on a fourth straight Serie A title after a 2-0 victory over Empoli.
Roma midfielder Radja Nainggolan is unsure whether he will remain with the Italian club next season.