Fiorentina ended Inter Milan's perfect record in stunning style while AC Milan were beaten and Lazio moved up to fourth.
Juventus could only draw 1-1 at home to Frosinone in Serie A on Wednesday, as leaders Inter Milan beat Hellas Verona 1-0.
A Gonzalo Higuain-inspired Napoli ran riot against Lazio while there were also wins for Inter Milan and Fiorentina.
Summer signing Paulo Dybala netted a late penalty to earn Juventus a 1-1 draw against Chievo.
Serie A champions Juventus saw their 47-match unbeaten home league record come to an end against Udinese.
Fiorentina finished their Serie A campaign on a five-game winning streak after beating 10-man Chievo 3-0.
Fiorentina qualified for the Europa League while Stephan El Shaarawy scored his first goal in seven months for AC Milan.
Liverpool give U21s more game time than Premier League rivals but top flight lags behind Europe.
Chievo remained unbeaten at home since February 2 after finding a late equaliser to draw 1-1 against Atalanta.
Torino retain an outside chance of sneaking into the Europa League after dominating Chievo to win 2-0 on Sunday.
Napoli's hopes of Champions League qualification suffered a setback as they drew 2-2 at rock-bottom Parma in Serie A on Sunday.
Chievo and Hellas Verona drew 2-2 in Sunday's derby with all of the goals scored in a thrilling first half.
Inter Milan were held to a goalless draw at home to Chievo on Sunday.
Chievo beat relegation-threatened Cagliari on Wednesday evening to ensure themselves of Serie A football next season.
Chievo striker Alberto Paloschi equalised 15 minutes from time to deny second-placed Lazio an important home win.
Bostjan Cesar's own goal cost Chievo an important Serie A win at home to Udinese on Sunday as they drew 1-1.
Domenico Berardi struck from the penalty spot to hand 10-man Sassuolo a 1-0 home triumph over Chievo.
Juventus closed in on a fourth straight Serie A title after a 2-0 victory over Empoli.
AC Milan beat Cagliari 3-1 at the San Siro to relieve some of the pressure on coach Filippo Inzaghi.
Chievo put plenty of daylight between themselves and the relegation zone thanks to a 1-0 win over Palermo on Saturday.