The CAF has rejected an appeal by Tunisia against a £33,795 fine for violent conduct after they were accused of bias.
Equatorial Guinea are through to the Africa Cup of Nations semi-finals after a dramatic 2-1 victory over Tunisia.
Yassine Chikhaoui scored a dramatic late winner to seal a 2-1 victory for Tunisia over Zambia in Africa Cup of Nations Group B.
Crystal Palace winger Yannick Bolasie earned DR Congo a 1-1 draw against Zambia after netting a second half equaliser.
Holders Nigeria failed to qualify for the 2015 Africa Cup of Nations after being held to a 2-2 draw by South Africa.
South Korea's World Cup warm-up preparations did not go to plan against Tunisia on Wednesday, as they lost 1-0.