A goal late in each half steered Heracles Almelo to three points as they beat NEC Nijmegen 2-0 at the Polman Stadion.
PSV Eindhoven remain third after only drawing 0-0 at Willem II on Saturday, while Excelsior, Heracles and Groningen all won.
Go Ahead Eagles ended Feyenoord's 23-game unbeaten streak in the Eredivisie after a famous 1-0 victory at De Adelaarshorst.
Hakim Ziyech's strike was enough as Ajax edged Excelsior with a 1-0 win to keep the pressure on Eredivisie leaders Feyenoord.
Feyenoord dropped points in the Eredivisie for the first time this season after a 1-1 draw with rivals Ajax.
Feyenoord maintained their winning start to the Eredivisie season at Willem II, while there was also a win for Ajax.