England's women suffered a two-wicket defeat against a Cricket Australia Representative XI in Melbourne.
Sarah Taylor top scored with 52 in England's total of 237 all out in the 50-over tour match but, despite three wickets each for Jenny Gunn and Danielle Hazell, the CA Representative XI chased down their target with 13 balls to spare.
England skipper Charlotte Edwards (39) put on 77 for the second wicket with Taylor to set a solid platform after the tourists were asked to bat in sweltering conditions at Junction Oval.
Lydia Greenway (44) and Natalie Sciver (42) upped the scoring rate with a 75-run stand in 12 overs but wickets tumbled late in the innings, Julie Hunter finishing with 3-30.
CA Representative XI opener Nicole Bolton set up the run chase with 46 off 47 deliveries before becoming the first of Hazell's wickets.
Jess Jonassen stroked 52 but Hazell (3-46) removed her and Sarah Elliott in successive balls to leave the home side on 184-6 in the 36th over.
Hunter and Megan Schutt fell cheaply to Gunn (3-41) in a tense finale but Kelly Applebee held her nerve to steer the CA Representative XI across the winning line with 29 not out.
England return to action at the MCG on Sunday for the first of three one-day internationals against Australia, which count towards the points-based Ashes format.
The tourists made a good start to their Ashes defence with victory in the lone Test by 61 runs at the WACA in Perth.
That earned them six points and means England require just two victories from the three ODIs and three Twenty20 matches over the next fortnight to retain the urn.