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Wigan Warriors will be gunning for a third straight win over the Widnes Vikings and a place in the Challenge Cup semi-finals when the pair meet at the DW Stadium on Monday.
The Warriors crushed Denis Betts' team 62-4 at home earlier in the season with Josh Charnley helping himself to four tries, before an under-strength Wigan side edged out the Vikings 33-32 in June.
The good news for the 2012 League Leaders' Shield winners is that star full-back Sam Tomkins is expected to win his battle against an Achilles injury and line up against the Vikings.
Captain Sean O'Loughlin is expected to miss out, although England prop Lee Mossop and centre Darrell Goulding are back for the Super League leaders.
Wigan were upset by the Leeds Rhinos in last year's semi-final at Huddersfield's John Smith's Stadium and Wane knows his troops must be on their game if they are to secure their place in the final four.
"We had 10 or 11 first teamers out that day so we are expecting a very different encounter on Monday, we weren't at our best, but we managed to get a win," Wane told Wigan's official website.
"This is a Cup game so it's what happens in the 80 minutes which is crucially important. On any day any one side can beat another so we have to turn up and play well.
"We will give Widnes the respect they deserve, our preparations have been spot on and we will be getting a couple of players back with Sam and Lee Mossop, so we are in a good position and we are looking forward to the game."
Former Wigan Warriors youngster Eamon O'Carroll is set to make his 100th appearance at club level in Monday night's clash, while on-loan Gareth Hock is prevented from playing against his parent club and will miss out.
Widnes are without former Warriors centre Cameron Phelps but expect to have half-back Joe Mellor back after missing his first match of the season at Wakefield last week and he will be one of six ex-Wigan players in their line-up.
Vikings captain Kevin Brown is one of those six and he is well aware of the task facing Widnes at the DW Stadium.
"There aren't many clubs who have a better cup pedigree than Widnes and it would be great if we could win the Challenge Cup to put in our trophy cabinet," Brown told Widnes' official website.
"The Challenge Cup is really special to me, some of my best career memories are playing in the competition and I'm hoping to add to those this year.
"All the attention is on Wigan, there's no pressure on us as everybody is writing us off. That's good for us as we can go there feeling relaxed and enjoy it, but make no mistake we are going there to win and we will give it our best shot.
"Wigan have been the best team in rugby league for the last few years but the beauty of the Challenge Cup is that it's one game and anything can happen."
Wigan Warriors: Charnley, Crosby, Dudson, Flower, Goulding, Burke, Hansen, Hughes, Tuson, Mossop, O'Loughlin, Green, Richards, Smith, Farrell, Gelling, Hampshire, S. Tomkins, McIlorum.
Widnes Vikings: Ah Van, Allen, K. Brown, Cahill, Clarke, Craven, Gerrard, Gore, Hanbury, Isa, Joseph, Kavanagh, Lawton, Leuluai, Marsh, Mellor, O'Carroll, Owens, Winterstein.
|Tetleys Challenge Cup|
|Friday 14th March|
|19:30||Wigan St Patricks vs Leigh|
|Saturday 15th March|
|14:00||Normanton Knights vs Workington|
|15:00||Egremont Rangers vs Oldham|
|Sunday 16th March|
|14:00||Milford Marlins vs Barrow|
|14:00||Sheffield vs East Leeds|
|14:00||Hull Dockers vs Halifax|
|14:00||North Wales Crusaders vs Pilkington Recs|
|15:00||Dewsbury vs West Hull|
|15:00||Rochdale vs Gloucestershire All Golds|
|15:00||Swinton vs Gateshead|
|Tetleys Challenge Cup|
|Sunday 23rd February|
|Great Britain Police 30 - 34 The Army|
|Saturday 22nd February|
|Rochdale Mayfield 28 - 36 Wigan St Patricks|
|Kells 26 - 12 Leigh Miners|
|Walney Central 16 - 28 Egremont Rangers|
|Normanton 44 - 10 Royal Air Force|
|Wath Brow 12 - 6 Elland|
|Hull Dockers 42 - 8 Siddal|
|East Leeds 28 - 16 Leeds Met Uni|
|South London 6 - 24 Milford|
|West Hull 28 - 18 Thatto Heath Crusaders|
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