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Ospreys host Harlequins at Brewery Field this Sunday with both teams still in with a chance of qualifying.
The home side must win and hope other results go their way; Harlequins need just two points to secure qualification.
Rhys Webb will skipper an Ospreys team that has been hit by injuries and international call-ups.
They do include Wales internationals Jonathan Thomas and five-time capped Kiwi Campbell Johnstone.
Rory Clegg returns at fly-half and Ross Chilsholm starts at fullback after coming back from injury last week.
Luke Wallace will lead the side as they aim for a home semi-final.
Ospreys: 15 Jamie Murphy, 14 Tom Grabham, 13 Jonathan Spratt, 12 Ben John, 11 Dafydd Howells, 10 Matthew Morgan, 9 Rhys Webb (c), 8 Morgan Allen, 7 Arthur Ellis, 6 Jonathan Thomas, 5 Sam Williams, 4 Lloyd Peers, 3 Campbell Johnstone, 2 Scott Baldwin, 1 Marc Thomas
Replacements: 16 Matthew Dwyer, 17 Duncan Jones, 18 Dmitri Arhip, 19 Ian Gough, 20 George Stowers, 21 Dan Baker, 22 Tom Habberfield, 23 Kah Fotuali'i
Harlequins: 15 Ross Chisholm, 14 Seb Stegmann, 13 Matt Hopper, 12 Ben Botica, 11 Sam Smith, 10 Rory Clegg, 9 Karl Dickson, 8 Tom Guest, 7 Luke Wallace (c), 6 Joe Trayfoot, 5 Charlie Matthews, 4 Sam Tworney, 3 Will Collier, 2 Dave Ward, 1 Mark Lambert
Replacements: 16 Henry Allen, 17 Darryl Marfo, 18 James Johnston, 19 Charlie Walker, 20 Maurie Fa'asavalu, 21 Jordan Burns, 22 Louis Grimoldby, 23 Ollie Lindsay-Hague
"Harlequins will provide a really difficult test for us. They aren't top of the Premiership and current champions, and top seeds in Europe for nothing. They have a quality squad and come to Bridgend looking for a result that could see them through to the top four so it's going to be a good game.
"It's a challenge but our preparation for the game has been good, the players know what is expected of them and that they need to be at their best if we are going to get the level of performance needed to get anything out of this match."
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