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Gloucester director of rugby Nigel Davies has made five changes to his starting XV for Friday's clash with defending Aviva Premiership champions Harlequins.
There is just one switch in the three-quarters as Henry Trinder comes in for Mike Tindall.
Up front, Koree Britton will make his first league start at hooker, while Lua Lokotui returns to form a second-row alliance with Jim Hamilton.
In the back row, Tom Savage and Akapusi Qera resume their usual positions as blindside and openside flanker respectively.
Davies acknowledged that his team will have to be at their best to take something from Quins, stating: "It's all about how we perform on Friday night. It's a big game, against a tough opponent who have been trailblazing over the last two or three years in terms of their performances and results. So, it'll be a big test for us."
With their trip to Kingsholm coming just five days after their last outing, Harlequins have made six changes to the side that lost to Saracens last Sunday.
In the backline, Ross Chisholm comes in for Ugo Monye, while George Lowe and Tom Casson combine in the centres in place of Jordan Turner-Hall and Matt Hopper.
In the pack, Rob Buchanan is named at hooker with Olly Kohn returning from a hamstring injury to start in the second row.
With Chris Robshaw being rested for this fixture, Luke Wallace joins Tom Guest and Nick Easter, who captains the team, in the back-row.
Gloucester: 15 Rob Cook, 14 Charlie Sharples, 13 Henry Trinder, 12 Billy Twelvetrees, 11 Jonny May, 10 Freddie Burns, 9 Dan Robson, 8 Sione Kalamafoni, 7 Akapusi Qera, 6 Tom Savage, 5 Jim Hamilton (c), 4 Lua Lokotui, 3 Shaun Knight, 2 Koree Britton, 1 Dan Murphy
Replacements: 16 Huia Edmonds, 17 Nick Wood, 18 Dario Chistolini, 19 Will James, 20 Andy Hazell, 21 Dave Lewis, 22 Mike Tindall, 23 Martyn Thomas.
Harlequins: 15 Mike Brown, 14 Ross Chisholm, 13 George Lowe, 12 Tom Casson, 11 Sam Smith, 10 Nick Evans, 9 Danny Care, 8 Nick Easter (c), 7 Luke Wallace, 6 Tom Guest, 5 George Robson, 4 Olly Kohn, 3 James Johnston, 2 Rob Buchanan, 1 Joe Marler
Replacements: 16 Joe Gray, 17 Mark Lambert, 18 Will Collier, 19 Charlie Matthews, 20 Joe Trayfoot, 21 Karl Dickson, 22 Ben Botica, 23 Matt Hopper
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|Friday 21st March|
|19:45||Worcester vs Wasps|
|Saturday 22nd March|
|14:00||Sale Sharks vs Northampton|
|15:00||Gloucester vs Newcastle|
|15:15||Saracens vs Harlequins|
|17:30||London Irish vs Bath|
|Sunday 23rd March|
|14:00||Leicester vs Exeter|
|Friday 28th March|
|19:45||Bath vs Sale|
|Saturday 29th March|
|15:00||Wasps vs Saracens|
|15:00||Harlequins vs London Irish|
|15:00||Exeter vs Gloucester|
|15:15||Northampton vs Leicester|
|Sunday 30th March|
|14:00||Newcastle vs Worcester|
|Friday 11th April|
|19:45||Sale vs Harlequins|
|Saturday 12th April|
|15:00||Worcester vs Exeter|
|15:00||Leicester vs Wasps|
|15:15||Gloucester vs Bath|
|Sunday 13th April|
|14:00||Saracens vs Northampton|
|15:00||London Irish vs Newcastle|
|Friday 18th April|
|19:45||Harlequins vs Leicester|
|Saturday 19th April|
|15:00||Bath vs Worcester|
|15:00||Exeter vs Sale Sharks|
|15:15||Wasps vs Gloucester|
|Sunday 20th April|
|14:00||Northampton vs London Irish|
|15:00||Newcastle vs Saracens|
|Saturday 3rd May|
|15:00||Bath vs Northampton|
|Sunday 2nd March|
|Newcastle 18 - 41 Leicester|
|Saturday 1st March|
|Northampton 39 - 13 Gloucester|
|Exeter 18 - 0 London Irish|
|Harlequins 21 - 20 Worcester|
|Wasps 17 - 21 Sale Sharks|
|Friday 28th February|
|Bath 10 - 23 Saracens|
|Sunday 23rd February|
|Saracens 23 - 10 Exeter|
|Newcastle 16 - 22 Northampton|
|London Irish 15 - 20 Leicester|
|Saturday 22nd February|
|Worcester 12 - 24 Sale Sharks|
|Bath 32 - 25 Wasps|
|Gloucester 25 - 20 Harlequins|
|Sunday 16th February|
|Leicester 11 - 8 Gloucester|
|Saturday 15th February|
|Exeter 23 - 27 Bath|
|Wasps 20 - 23 London Irish|
|Northampton 30 - 14 Worcester|
|Harlequins 18 - 14 Newcastle|
|Friday 14th February|
|Sale Sharks 10 - 15 Saracens|
|Sunday 9th February|
|Harlequins 11 - 10 Wasps|
|Saturday 8th February|
|Exeter 16 - 17 Northampton|
|Saracens 13 - 22 London Irish|
|Bath 24 - 6 Newcastle|
|Friday 7th February|
|Sale Sharks 24 - 19 Gloucester|
|Worcester 22 - 23 Leicester|
Read the thoughts and opinions of Stuart Barnes with skysports.com