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Gloucester director of rugby Nigel Davies has made five changes to his starting XV for Friday's clash with defending Aviva Premiership champions Harlequins.
There is just one switch in the three-quarters as Henry Trinder comes in for Mike Tindall.
Up front, Koree Britton will make his first league start at hooker, while Lua Lokotui returns to form a second-row alliance with Jim Hamilton.
In the back row, Tom Savage and Akapusi Qera resume their usual positions as blindside and openside flanker respectively.
Davies acknowledged that his team will have to be at their best to take something from Quins, stating: "It's all about how we perform on Friday night. It's a big game, against a tough opponent who have been trailblazing over the last two or three years in terms of their performances and results. So, it'll be a big test for us."
With their trip to Kingsholm coming just five days after their last outing, Harlequins have made six changes to the side that lost to Saracens last Sunday.
In the backline, Ross Chisholm comes in for Ugo Monye, while George Lowe and Tom Casson combine in the centres in place of Jordan Turner-Hall and Matt Hopper.
In the pack, Rob Buchanan is named at hooker with Olly Kohn returning from a hamstring injury to start in the second row.
With Chris Robshaw being rested for this fixture, Luke Wallace joins Tom Guest and Nick Easter, who captains the team, in the back-row.
Gloucester: 15 Rob Cook, 14 Charlie Sharples, 13 Henry Trinder, 12 Billy Twelvetrees, 11 Jonny May, 10 Freddie Burns, 9 Dan Robson, 8 Sione Kalamafoni, 7 Akapusi Qera, 6 Tom Savage, 5 Jim Hamilton (c), 4 Lua Lokotui, 3 Shaun Knight, 2 Koree Britton, 1 Dan Murphy
Replacements: 16 Huia Edmonds, 17 Nick Wood, 18 Dario Chistolini, 19 Will James, 20 Andy Hazell, 21 Dave Lewis, 22 Mike Tindall, 23 Martyn Thomas.
Harlequins: 15 Mike Brown, 14 Ross Chisholm, 13 George Lowe, 12 Tom Casson, 11 Sam Smith, 10 Nick Evans, 9 Danny Care, 8 Nick Easter (c), 7 Luke Wallace, 6 Tom Guest, 5 George Robson, 4 Olly Kohn, 3 James Johnston, 2 Rob Buchanan, 1 Joe Marler
Replacements: 16 Joe Gray, 17 Mark Lambert, 18 Will Collier, 19 Charlie Matthews, 20 Joe Trayfoot, 21 Karl Dickson, 22 Ben Botica, 23 Matt Hopper
|Click here for more betting|
|Friday 2nd May|
|19:45||Bath vs Northampton|
|Saturday 3rd May|
|15:00||Saracens vs Worcester|
|15:00||Wasps vs Newcastle|
|15:00||Gloucester vs London Irish|
|15:15||Sale vs Leicester|
|Sunday 4th May|
|14:00||Exeter vs Harlequins|
|Saturday 10th May|
|15:15||Newcastle vs Exeter|
|15:15||Northampton vs Wasps|
|15:15||Worcester vs Gloucester|
|15:15||Leicester vs Saracens|
|15:15||Harlequins vs Bath|
|15:15||London Irish vs Sale|
|Sunday 20th April|
|Newcastle 18 - 23 Saracens|
|Northampton 36 - 21 London Irish|
|Saturday 19th April|
|Wasps 38 - 30 Gloucester|
|Bath 32 - 20 Worcester|
|Exeter 12 - 55 Sale|
|Friday 18th April|
|Harlequins 24 - 20 Leicester|
|Sunday 13th April|
|London Irish 40 - 12 Newcastle|
|Saracens 28 - 24 Northampton|
|Saturday 12th April|
|Gloucester 17 - 18 Bath|
|Worcester 33 - 38 Exeter|
|Leicester 27 - 15 Wasps|
|Friday 11th April|
|Sale Sharks 12 - 27 Harlequins|
|Sunday 30th March|
|Newcastle 12 - 17 Worcester|
|Saturday 29th March|
|Northampton 16 - 22 Leicester|
|Wasps 20 - 32 Saracens|
|Exeter 13 - 14 Gloucester|
|Harlequins 23 - 9 London Irish|
|Friday 28th March|
|Bath 11 - 12 Sale Sharks|
|Sunday 23rd March|
|Leicester 45 - 15 Exeter|
|Saturday 22nd March|
|London Irish 23 - 44 Bath|
|Saracens 39 - 17 Harlequins|
|Gloucester 40 - 33 Newcastle|
|Sale Sharks 19 - 6 Northampton|
|Friday 21st March|
|Worcester 11 - 13 Wasps|
Read the thoughts and opinions of Stuart Barnes with skysports.com