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Defending champions Toulouse have made the Top 14 semi-finals every year since 1994 but will need to be at the top of their game to stop Racing-Metro ended their sequence.
Racing have won 11 of their last 13 matches while Toulouse have not played like champions, losing nine matches - their worst record for four years.
Toulouse are missing try-magnet Vincent Clerc due to a serious knee injury as well as France centre Florian Fritz.
However, number eight Louis Picamoles has been passed fit while wing Maxime Médard is included in the starting line-up.
Racing are without France wing Benjamin Fall as he recovers from a thigh injury which forced him to miss the last league match, although winger Juan Imhoff has been cleared to play.
Racing-Metro's good end-of-season form belies the huge internal ructions at the club owned by Swiss property magnate Jacky Lorenzetti.
Victory for the hosts will bring to an end the stormy reign of former France and Italy coach - and managing director for the past season - Pierre Berbizier, who will leave the club having already been removed involvement in coaching the side.
Not only will Berbizier be departing but also their Argentinian coach Gonzalo Quesada - for bitter Parisian rivals Stade Français.
Stade Toulousain: 15 Clement Poitrenaud, 14 Yoann Huget, 13 Yannick Jauzion, 12 Gael Fickou, 11 Maxime Medard, 10 Luke McAlister, 9 Jean Marc Doussain, 8 Louis Picamoles, 7 Thierry Dusautoir, 6 Jean Bouilhou, 5 Patricio Albacete, 4 Yoann Maestri, 3 Census Johnston, 2 William Servat , 1 Vasil Kakovin
Replacements: 16 Jaba Bregvadze, 17 Gurthro Steenkamp, 18 Romain Millo chlusky, 19 Yannick Nyanga, 20 Luke Burgess, 21 Lionel Beauxis, 22 Yann David, 23 Jean Batiste Poux.
Racing-Métro 92: 15 Juan Martin Hernandez, 14 Juan Imhoff, 13 Henry Chavancy, 12 Fabrice Estebanez, 11 Sereli Bobo, 10 Jonathan Wisniewski, 9 Maxime Machenaud, 8 Masinivanua Matadigo, 7 Bernard le Roux, 6 Antoine Battut, 5 Francois van der Merwe, 4 Manuel Carizza, 3 Luc Ducalcon, 2 Dimitri Szarzewski, 1 Julien Brugnaut.
Replacements: 16 Thomas Bianchin, 17 Eddy Ben arous, 18 Alvaro Galindo, 19 Jacques Cronje, 20 Sebastien Descons, 21 Alexandre Dumoulin, 22 Virimi Vakatawa, 23 Salemane Sa.
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|Saturday 19th April|
|Stade Francais 37 - 23 Bordeaux-Begles|
|Biarritz 19 - 13 Brive|
|Racing Metro Paris 22 - 6 Clermont Auvergne|
|Grenoble 21 - 21 Bayonne|
|Perpignan 31 - 46 Toulon|
|Oyonnax 19 - 19 Toulouse|
|Friday 18th April|
|Castres 22 - 15 Montpellier|
|Saturday 12th April|
|Toulouse 16 - 9 Brive|
|Bayonne 24 - 19 Stade Francais|
|Grenoble 30 - 36 Montpellier|
|Racing Metro Paris 37 - 7 Biarritz|
|Perpignan 22 - 12 Oyonnax|
|Bordeaux-Begles 20 - 22 Toulon|
|Friday 11th April|
|Clermont Auvergne 23 - 11 Castres|
|Saturday 5th April|
|Oyonnax 26 - 12 Bordeaux-Begles|
|Saturday 29th March|
|Toulon 32 - 28 Toulouse|
|Biarritz 34 - 34 Castres|
|Bordeaux-Begles 23 - 5 Perpignan|
|Oyonnax 40 - 13 Grenoble|
|Montpellier 43 - 27 Bayonne|
|Stade Francais 22 - 32 Racing Metro Paris|
|Friday 28th March|
|Brive 26 - 24 Clermont Auvergne|
|Saturday 22nd March|
|Grenoble 13 - 26 Racing Metro Paris|
|Bayonne 22 - 23 Bordeaux-Begles|
|Oyonnax 8 - 22 Montpellier|
Read the thoughts and opinions of Stuart Barnes with skysports.com