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Toulon booked their place in the Top 14 play-offs with a narrow win at home to defending champions Toulouse on Saturday.
A losing bonus point was enough for Toulouse to retain the championship lead however, as Clermont lost on the road to Castres.
Racing join Castres in the play-offs after eliminating Parisian rivals Stade Français from the race in a tight battle in the capital.
Brive are now favourites to be relegated with Lyon after losing heavily at home to Bordeaux-Bègles while Bayonne won with a bonus point against Agen.
Elsewhere, Perpignan ensured their survival with a bad-tempered win over Lyon.
On Friday, Biarritz also secured their place in the top division next season by getting a losing bonus point in Montpellier.
RC Toulon 25-22 Stade Toulousain
A huge result for Toulon who came from behind to beat a near-full-strength Toulouse side and virtually assure a home play-off in the process.
Toulouse have now lost six of their last nine games in all competitions despite having started the match in great style, taking a 16-8 lead into the half-time break thanks to a try from Vincent Clerc.
It seemed like Toulouse had got their mojo back as they were playing great rugby, but Toulon fought back in the second period with Steffon Armitage continuing his strong recent form with the winning try in the 65th minute.
Lionel Beauxis and Jonny Wilkinson traded early penalties in a pulsating match played in perfect conditions, the Toulouse man hitting a second before the home side hit back.
Toulon centre Matt Giteau made a scything break through a porous defence before sending out an inch-perfect pass to Alexis Palisson, the France winger crossing in the corner for a try Wilkinson failed to convert.
Clerc responded with a well-taken try after a similar miss-pass from Beauxis, who nailed the extras and a 33rd minute penalty for a 16-8 Toulouse lead.
Welsh prop Eifion Lewis-Roberts was bundled over for a timely converted try early in the second-half for the hosts before Beauxis and Wilkinson again traded penalties.
Armitage scored the decisive five-pointer, jinking through under the posts after a period of sustained pressure.
Wilkinson converted to make it 25-19 with 15 minutes to play, Beauxis hitting a penalty shortly after, but the home side held out for the victory.
Aviron Bayonnais 31-10 SU Agen
Bayonne's expensively assembled squad finally produced the goods, outscoring their visitors four tries to one to move within touching distance of safety.
Bayonne waited for the second last round of the championship to record their first bonus point win and climb out of the relegation zone, five points clear of Brive.
Brive's failure to get a bonus point means that Agen are also safe.
Agen led 10-7 thanks to a try from full-back Brice Dulin, who cancelled out Bayonne centre Sam Gerber's touchdown.
But the Bayonne pack dominated the second half, winning a penalty try and setting up two more.
CA Brive Corrèze 9-23 Union Bordeaux-Bègles
Brive are almost certain to be relegated after failing to come up with the goods when it mattered most.
Now five points adrift of Bayonne, Brive now face the prospect of not only having to win with a bonus point in their final away game at heavyweights Clermont, but also need Bayonne to lose, without a defensive bonus point, away to Castres.
Castres Olympique 30-19 ASM Clermont Auvergne
Castres booked their place in the play-offs by inflicting only Clermont fifth loss of the season.
One point behind Montpellier, CO will also fancy their chances of bagging a home play-off next week against Bayonne as Montpellier must visit Toulouse.
Castres full-back Romain Teulet become the first man in world rugby to score 3000 points for a single club with the game's opening penalty.
Clermont sent a second-string team but still managed to open the scoring via a try from lock Loic Jacquet.
But Castres were 17-7 ahead at half time tanks to tries from flank Ibrahim Diarra and centre Max Evans.
A second-half try from Romain Martial proved enough for victory. USA Perpignan 34-22 Lyon Olympique
Mission accomplished for USAP, who assured their place in the Top 14 next season, despite playing nearly an hour with a man down after hooker Guilhem Guirado was red carded for a tip tackle.
It was an emotional day for Perry Freshwater, Olivier Olibeau, Marius Tincu, Grégory Le Corvec, Rimas Alvarez-Kairelis, Damien Chouly and Gerrie Britz, who all played their last game at Stade Aimé Giral.
Two excellent tries from Adrien Planté and David Marty helped USAP a 23-12 lead at half time before Chouly sealed the win in the second period.
Mickael Forest's late consolation try for Lyon denied Perpignan a bonus point but the Catalans still climbed to eighth in the standings.
Racing-Métro 19-13 Stade Français Paris
In the season's second Paris derby, Racing took revenge for their heavily loss at the same venue in Round 12.
This time around Pierre Berbizier's men clinched a crucial victory that leaves Stade Français five points adrift of the play-off berths and needing a minor miracle to make the top six.
Stade Français took the early lead via a try from wing Julien Arias but Racing were 13-10 ahead at the break thanks to scrum-half Sebastien Descons's touchdown just before the break when Stade were a man down after Felipe Contepomi was sent to the bin for playing the ball on the ground.
Two second-half penalties from full-back Jonathan Wisniewski were enough to secure victory for Racing.
|Saturday 11th May|
|Castres 25 - 12 Montpellier|
|Friday 10th May|
|Toulouse 33 - 19 Racing Metro Paris|
|Stade Francais 19 - 16 Racing Metro Paris|
|Saturday 4th May|
|Biarritz 52 - 17 Stade Francais|
|Clermont Auvergne 67 - 3 Bordeaux-Begles|
|Montpellier 50 - 22 Perpignan|
|Racing Metro Paris 29 - 28 Castres|
|Mont de Marsan 33 - 36 Bayonne|
|Toulon 43 - 21 Agen|
|Toulouse 57 - 7 Grenoble|
|Saturday 20th April|
|Grenoble 25 - 24 Toulon|
|Agen 23 - 15 Perpignan|
|Bayonne 32 - 26 Montpellier|
|Bordeaux-Begles 16 - 0 Biarritz|
|Castres 44 - 17 Mont de Marsan|
|Clermont Auvergne 39 - 17 Toulouse|
|Sunday 14th April|
|Toulon 26 - 26 Clermont Auvergne|
|Perpignan 33 - 28 Biarritz|
|Saturday 13th April|
|Agen 20 - 28 Stade Francais|
|Bayonne 31 - 24 Grenoble|
|Montpellier 10 - 8 Toulouse|
|Friday 12th April|
|Castres 23 - 23 Bordeaux-Begles|
Read the thoughts and opinions of Stuart Barnes with skysports.com