The Premier League have admitted their concern after it emerged junior matches can now be gambled on.
SBObet and 188bet, who are both licensed on the Isle of Man, offer live betting on matches at Premier League under-18 level and similar matches across Europe including the German Bundesliga under-17 league.
Both betting firms have a presence in the Premier League through tie-ups with several leading clubs.
SBObet are shirt sponsors of West Ham, while 188bet sponsor Bolton and Wigan and are the official gaming partner of Chelsea and official betting partner of Aston Villa.
Gambling on junior matches does not contravene any rules, but the Premier League will examine the issue and have pledged to ensure football's relationship with the betting industry is properly regulated.
"We are alarmed at these developments and are currently discussing this matter with our clubs and the government," a Premier League spokesman told the Sunday Telegraph.
"What is clear is that this further highlights the need for the relationship between sport and the gambling industry to be properly regulated in order to protect the integrity of sport."
Professional Footballers Association chief executive Gordon Taylor said: "It is making our youngsters vulnerable, without a shadow of a doubt. Youngsters and gambling is a bad mix, and an unhealthy one."
A spokesman for 188bet told the newspaper that the company is committed to protecting 'betting integrity' and stressed they were unaware of any concerns from the Premier League or PFA.
The spokesman said: "A key element of our betting governance includes setting lower bet limits on smaller markets, such as Premier League Academy games, to ensure that maximum individual bets and total bet volume is not of a size that would influence any sporting outcome."

Latest Sky Sports News Report
Paper review - 10th February
Chelsea v Arsenal
Birmingham v Wolves
Portsmouth v Sunderland - 1st Half
Wigan v Stoke - 2nd Half
Wigan v Stoke - 1st Half
Fulham v Burnley - 2nd Half
| Time | Fixture |
|---|---|
| Wednesday 10th February | |
| Barclays Premier League | |
| 19:45 | Arsenal vs Liverpool |
| 19:45 | Aston Villa vs Man Utd |
| 19:45 | West Ham vs Birmingham |
| 19:45 | Wolverhampton vs Tottenham |
| 20:00 | Blackburn vs Hull |
| 20:00 | Everton vs Chelsea |
| Tuesday 16th February | |
| Barclays Premier League | |
| 19:45 | Stoke vs Man City |
| Wednesday 17th February | |
| Barclays Premier League | |
| 20:00 | Wigan vs Bolton |
| Saturday 20th February | |
| Barclays Premier League | |
| 12:45 | Everton vs Man Utd |
| 15:00 | Arsenal vs Sunderland |
| 15:00 | West Ham vs Hull |
| 15:00 | Wolverhampton vs Chelsea |
| 17:30 | Portsmouth vs Stoke |
| Sunday 21st February | |
| Barclays Premier League | |
| 14:00 | Aston Villa vs Burnley |
| 15:00 | Fulham vs Birmingham |
| 15:00 | Man City vs Liverpool |
| 16:15 | Wigan vs Tottenham |
| Monday 22nd February | |
| Barclays Premier League | |
| 20:00 | Blackburn vs Bolton |
| Tuesday 23rd February | |
| Barclays Premier League | |
| 20:00 | Man Utd vs West Ham |
| Saturday 27th February | |
| Barclays Premier League | |
| 12:45 | Chelsea vs Man City |
| Time | Result |
|---|---|
| Tuesday 9th February | |
| Barclays Premier League | |
| Man City 2 - 0 Bolton | |
| Wigan 1 - 1 Stoke | |
| Portsmouth 1 - 1 Sunderland | |
| Fulham 3 - 0 Burnley | |
| Sunday 7th February | |
| Barclays Premier League | |
| Birmingham 2 - 1 Wolverhampton | |
| Chelsea 2 - 0 Arsenal | |
| Saturday 6th February | |
| Barclays Premier League | |
| Liverpool 1 - 0 Everton | |
| Hull 2 - 1 Man City | |
| Stoke 3 - 0 Blackburn | |
| Bolton 0 - 0 Fulham | |
| Sunderland 1 - 1 Wigan | |
| Man Utd 5 - 0 Portsmouth | |
| Burnley 2 - 1 West Ham | |
| Tottenham 0 - 0 Aston Villa | |
| Wednesday 3rd February | |
| Barclays Premier League | |
| Fulham 1 - 0 Portsmouth | |
| Tuesday 2nd February | |
| Barclays Premier League | |
| Hull 1 - 1 Chelsea | |
| Monday 1st February | |
| Barclays Premier League | |
| Sunderland 0 - 0 Stoke | |
| Sunday 31st January | |
| Barclays Premier League | |
| Man City 2 - 0 Portsmouth | |
| Arsenal 1 - 3 Man Utd | |
| Saturday 30th January | |
| Barclays Premier League | |
| Birmingham 1 - 1 Tottenham | |
Portsmouth left it late to grab a point while Manchester City and Fulham were comfortable winners.
Norwich's success this season means more to Chris Martin than most, as Chris Burton found out.
See all the goals and major incidents involving YOUR team, right here on skysports.com.
David Sullivan has dismissed reports that Gianfranco Zola could be dismissed if West Ham lose to Birmingham.
Portsmouth are attempting to secure an adjournment in hearing their appeal against a winding-up petition.
Arsene Wenger has rubbished fresh reports linking Cesc Fabregas with a summer switch to Barcelona.
Nani says he never wanted to leave Man Utd during the difficult times he has experienced at Old Trafford.
John Terry's relationship with Fabio Capello will not have been damaged, according to Carlo Ancelotti.