Tottenham defender Danny Rose has withdrawn from the England squad to face Lithuania and Italy through injury.
Rose was called into the squad on Saturday after Luke Shaw was forced out with a hamstring problem but has now been sent back to Spurs with hip and hamstring injuries of his own.
An FA statement read: "Tottenham Hotspur's Danny Rose has withdrawn from the England camp through injury.
"The left-back will play no further part in preparation for the forthcoming fixtures against Lithuania and Italy and has returned to his club."
After impressing this season at club level, Rose was in line to make his full England debut in either the European qualifier against Lithuania on Friday or the friendly in Turin four days later.
But the 24-year-old has become the the fifth player to pull out of the international double-header since Roy Hodgson announced his squad last week.
Southampton goalkeeper Fraser Forster and Manchester United left-back Shaw withdrew on Saturday and they were followed on Monday by Liverpool pair Daniel Sturridge and Adam Lallana.
Everton's Leighton Baines now looks set to start at left-back in both matches provided he does not sustain an injury against the Lithuanians at Wembley.
That there has been no replacement for Rose announced comes as something of a surprise with Ryan Bertrand enjoying a solid season with Southampton.