Neil Harris will check on the fitness of Lee Gregory ahead of Millwall's League One play-off semi-final first leg against Scunthorpe, live on Sky Sports on Thursday.
The striker will be monitored by the manager after being forced off on Sunday due to illness.
Harris has no new injury or suspension worries but will assess several other players who have been affected by the sickness bug.
Despite concerns over his availability, Gregory has warned Scunthorpe that Thursday's visit could be a daunting experience.
The Lions, who clinched a top-six finish in thrilling fashion at Bristol Rovers on Sunday, have already beaten Premier League sides Bournemouth, Watford and Leicester on home soil in the FA Cup this season.
"We know we can beat any team at The Den," Gregory told the club's official website: "We've beaten three Premier League teams and it's a difficult place for our opposition to visit.
"Hopefully we can do the job at home and follow it up at their place on Sunday."
Millwall had to win at Bristol Rovers on the final day of the regular season to secure a play-off place and feared the worst when the home side equalised to make it 3-3 deep in the second half.
But defender Shaun Hutchinson's late header sealed a dramatic victory for the Lions and Gregory believes that could also prove significant.
"It was nuts, absolutely crazy," the 17-goal forward added. "The lads have come in absolutely buzzing this week.
"To make it through in the fashion that we did, winning 4-3 away from home, is a fantastic feeling and we can't wait for Thursday.
"To be honest, I thought we may have blown our chance when Bristol Rovers scored a late equaliser, but we've proved we can dig deep and get a late winner when it matters."
Scunthorpe boss Graham Alexander, whose side missed out on automatic promotion by four points after spending long periods in the top two, believes his players can cope with the partisan atmosphere expected in London.
"Over the course of the last 10 months we've played a lot of big games in big atmospheres, and I think we've handled it really well," he told the club's official website.
"We've got to cut out the nature of the game, the prize at stake, the atmosphere, and we've got to focus on the facts."
The Iron have bounced back from six defeats in eight matches heading into April with five straight wins and Alexander is confident his players will not be overawed by the occasion.
"We have to make sure we continue doing what we have been over the last month or so," he added.
"We've been really positive in our mindset and the players have played with great freedom, even though there was a lot of pressure on those games. That stands us in good stead."
Alexander, who has no new injury problems, will make a late decision on right-back Scott Wiseman, who missed Sunday's home win against Coventry due to a knee injury.
Millwall will be competing in the League One play-offs for the second consecutive season after their 2015-16 defeat to Barnsley in the final.
The Iron's last foray into the play-offs was in the 2008-09 League One campaign - they beat Millwall 3-2 in the final at Wembley.
Only champions Sheffield United (92) scored more goals than Scunthorpe in League One this season (80).
Millwall scored a league-high 11 goals from penalties in League One this season.
Josh Morris (19) and Lee Gregory (17) were the third and fourth top goalscorers in League One this season.
Scunthorpe scored six goals from direct free-kicks this season, the highest of any side in League One.
Millwall have failed to score in three of their last four league matches with Scunthorpe (W1 D1 L2).
Millwall have lost eight of their last 12 league matches against Scunthorpe, including the 2009 play-off final (W3 D1).
It will be tough for Scunny but I don't think they will be overawed going to The Den. As much as that ground has its own flavour, Scunny certainly has its own character too.
Both sides arrive into the match in decent form and I think this heads back to the second leg with all to play for.
PRUTTON PREDICTS: 1-1 (Sky Bet odds 11/2)