Phil Jones has been banned for two matches and Daley Blind fined by UEFA after the Manchester United pair were found guilty of breaching the governing body's doping regulations after the Europa League final in May.
Jones was charged for "insulting and directing abusive language" towards a doping control officer after he was requested to attend drug testing after the final in Stockholm on May 24.
He will now miss the UEFA Super Cup match against Real Madrid next week and United's first Champions League group stage match in September. He has also been fined €5,000 (£4,475).It is understood Blind was reluctant to take a test as requested because he wanted to celebrate with his team-mates, whose 2-0 victory over Ajax ensured their qualification for this season's Champions League. He was fined €5,000 (£4,475).
There is no suggestion that either player was guilty of doping.
United have been fined €10,000 (£8,950) over the rule breaches.
The club are understood to be aware of UEFA's decisions and have three days to appeal against them. However, they have not yet indicated to UEFA whether they will or not.
Blind played the full 90 minutes against his old club Ajax, while Jones was an unused substitute in Sweden.
United manager Jose Mourinho's defensive options in Europe had already been depleted after UEFA gave Eric Bailly an additional two-match ban for his red card against Celta Vigo earlier in the Europa League campaign.
Bailly missed the final in Stockholm, but must now also sit out the Super Cup and United's opening Champions League group stage match.
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UEFA announced the charges and sanctions on its website on Monday.
In its statement, it said: "The club [United] has been charged for infringements of the UEFA anti-doping regulations [ADR] concerning the doping control procedure.
"In particular, according to Appendix B (17) of the aforementioned regulations, 'the teams are responsible for ensuring that the players drawn to undergo doping controls are taken by the respective team representatives to the doping control station straight from the pitch as soon as the match is over. This applies even when chaperones are appointed by UEFA'.
"The player Philip Jones has been charged for insulting and directing abusive language towards the doping control officer (Article 15 (1) of the UEFA disciplinary regulations), as well as for lack of co-operation and respect towards the doping control procedure (Article 6.05 ADR).
"The player Daley Blind has been charged for violation and non-compliance with Article 6.05 (a) of the UEFA anti-doping regulations, which states that 'every player designated to undergo as doping control is personally responsible for reporting immediately to the doping control station as notified'."