Liverpool midfielder Curtis Jones and Tottenham defender Japhet Tanganga have been handed their first England Under-21 call-ups.
The pair have been selected for the Young Lions' Euro 2021 qualifiers in Andorra and at home to Turkey.
There is a recall for Burnley's Dwight McNeil after he was left out of last month's squad, while Norwich's Ben Godfrey is included ahead of his £30m move to Everton.
Rhian Brewster is also in, with the forward close to a £23.5m switch from Liverpool to Sheffield United.
Aidy Boothroyd's side need four points to secure qualification for next year's European U21 Championship finals tournament in Hungary and Slovenia.
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England U21s travel to Andorra on Wednesday, October 7 before hosting Turkey at Molineux on Tuesday, October 13.
Last month, Boothroyd's side beat Kosovo 6-0 and Austria 2-1 and sit top of Group Three, six points clear of Austria with a game in hand.
England U21 squad in full:
Goalkeepers: Ellery Balcombe (Brentford), Josef Bursik (Stoke City FC, on loan at Doncaster Rovers), Aaron Ramsdale (Sheffield United)
Defenders: Max Aarons (Norwich City), Ben Godfrey (Norwich City), Marc Guehi (Chelsea, on loan at Swansea), Reece James (Chelsea), James Justin (Leicester City), Tariq Lamptey (Brighton & Hove Albion), Jonathan Panzo (Dijon), Japhet Tanganga (Tottenham Hotspur)
Midfielders: Jude Bellingham (Borussia Dortmund), Josh Dasilva (Brentford), Tom Davies (Everton), Ebere Eze (Crystal Palace), Curtis Jones (Liverpool), Oliver Skipp (Tottenham Hotspur, on loan at Norwich City)
Forwards: Rhian Brewster (Liverpool), Callum Hudson-Odoi (Chelsea), Dwight McNeil (Burnley), Eddie Nketiah (Arsenal), Reiss Nelson (Arsenal), Ryan Sessegnon (Tottenham Hotspur)
