England manager Gareth Southgate says he will continue to give opportunities to younger players, with Joe Gomez and Harry Winks set to start against Spain.
Southgate confirmed Gomez will come into the side to replace John Stones, who is suspended for Monday's game after picking up a yellow card during the 0-0 draw with Croatia on Friday.
Jordan Henderson was also ruled out of the Spain match after being booked during the goalless draw in Rijeka, and Sky sources understand Tottenham midfielder Harry Winks will start in his place for the match in Seville.
"It's probably pretty clear Joe Gomez will be coming in for John [Stones]," said Southgate. "He's in really good form for his club and his matches for us against Spain and Brazil have been really polished.
"He's a young defender but he is a very good defender, good athleticism, uses the ball well. I'm looking forward to seeing him in this game. It's another opportunity for us to look at younger players.
"We're slowly building a squad of players who are comfortable playing at the highest level."
England already trail Spain by five points in Group A4 after being held by Croatia, and Southgate is expecting another tough outing against Luis Enrique's side, who beat Wales 4-1 in a friendly earlier this week.
"It's as difficult a test as you can have in European football at the moment, if not world football," said Southgate.
"But we're ready."