A goal late in each half steered Heracles Almelo to three points as they beat NEC Nijmegen 2-0 at the Polman Stadion.
PSV Eindhoven remain third after only drawing 0-0 at Willem II on Saturday, while Excelsior, Heracles and Groningen all won.
Go Ahead Eagles ended Feyenoord's 23-game unbeaten streak in the Eredivisie after a famous 1-0 victory at De Adelaarshorst.
Hakim Ziyech's strike was enough as Ajax edged Excelsior with a 1-0 win to keep the pressure on Eredivisie leaders Feyenoord.
Feyenoord dropped points in the Eredivisie for the first time this season after a 1-1 draw with rivals Ajax.
Feyenoord maintained their winning start to the Eredivisie season at Willem II, while there was also a win for Ajax.
Davinson Sanchez and Kasper Dolberg both scored twice as Ajax beat Zwolle 5-1 while PSV won at Excelsior.
Eric Botteghin struck a late winner as Feyenoord maintained their perfect start to the Eredivisie season with a 1-0 win at PSV.
Luuk de Jong capped a 24-minute blitz as PSV stormed back to the top of the Eredivisie with a 4-1 win over NEC in Nijmegen.
PSV Eindhoven surrendered their 100 per cent record in disappointing fashion after being held to a 0-0 draw at home to Groningen.
Wolves have signed Dutch striker Paul Gladon from Heracles Almelo for an undisclosed transfer fee.
Feyenoord continued their perfect start to the season and returned to the top of the Eredivisie with a 1-0 win at Heracles Almelo.
Eredivisie champions PSV made it two wins from two as Hector Moreno's goal secured a 1-0 victory over AZ Alkmaar, on Sunday.
Eljero Elia's hat-trick helped Feyenoord win 5-0 at FC Groningen on the opening weekend of the Eredivisie.
Ajax moved a step closer to reclaiming the Eredivisie title on Sunday with a thumping 4-0 win over FC Twente.
Ajax have returned to the top of the Eredivisie table after winning 2-0 away at Heerenveen.
PSV Eindhoven returned to the top of the Eredivisie table after a 3-0 success at Roda JC sealed a third straight win.
Davy Klaassen's goal handed Ajax a 1-0 win at bottom club SC Cambuur on Saturday that maintained their two-point lead at the top of the Eredivisie.
Marko van Ginkel scored twice as PSV returned to the top of the Eredivisie table with a 4-2 win at AZ Alkmaar on Saturday.
Ajax failed to take advantage of PSV's Saturday slip-up as they dropped their first points in five Eredivisie games at home to NEC.