National coach Inaki Saez has omitted the former Sevilla star in order to give him more time to settle into life in The Premiership with Arsenal.
While Reyes will remain in North London, Barcelona midfielder Xavi has come back into the squad for the match as his replacement.
Atletico Madrid's young striker Fernando Torres may return to the action for his club at the weekend, but he will not be risked in the friendly squad, and Espanyol's Raul Tamudo has been drafted in to play.
Tamudo will receive a great reception from the home crowd as the game is behind staged at his club Espanyol's Estadio Montjuic in Barcelona.
Real Madrid's Guti and Deportivo's Cesar are the only two other names to be missing from the squad that won a place in Portugal with a Euro 2004 play-off victory over Norway.
Full Spain squad:
Iker Casillas (Real Madrid), Santiago Canizares (Valencia), Carles Puyol (Barcelona), Ivan Helguera (Real Madrid), Carlos Marchena (Valencia), Michel Salgado (Real Madrid), Raul Bravo (Real Madrid), Joseba Etxeberria (Athletic Bilbao), Joaquin (Real Betis), Juan Carlos Valeron (Deportivo La Coruna), Xabi Alonso (Real Sociedad), Ruben Baraja (Valencia), Vicente (Valencia), David Albelda (Valencia), Xavi (Barcelona), Raul (Real Madrid), Raul Tamudo (Espanyol), Albert Luque (Deportivo Coruna).
