Spain coach Vicente del Bosque has called up in-form Sevilla midfielder Vitolo for the first time for the holders' Euro 2016 qualifier at home to Ukraine next Friday, while Chelsea forward Diego Costa returns to the squad.
Vitolo, 25, typically operates in an attacking role down the left and as well as scoring goals is a regular provider of assists.
The former Spain Under-19 international makes his debut in the senior squad along with Villarreal goalkeeper Sergio Asenjo, who also featured for the Iberian nation at junior level.
Brazil-born Costa missed Spain's final two matches of 2014, a 3-0 qualifying win at home to Belarus and a 1-0 defeat to world champions Germany.
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He will compete with a place up front with Alvaro Morata of Juventus and Barcelona's Pedro, while Chelsea midfielder Cesc Fabregas was also named as a forward in the 24-man squad.
With four matches played, Spain are second in qualification Group C on nine points, three behind leaders Slovakia, who beat them 2-1 in Zilina in October, and level with Ukraine.
Spain also play a friendly against Netherlands in Amsterdam on March 31, a repeat of the 2010 World Cup final and the first time the pair meet since Netherlands crushed Spain 5-1 in the group stage at the 2014 World Cup.
Full squad for Euro 2016 qualifer
Goalkeepers: Iker Casillas, David De Gea, Sergio Asenjo
Defenders: Dani Carvajal, Gerard Pique, Marc Bartra, Juanfran, Raul Albiol, Sergio Ramos, Jordi Alba, Juan Bernat
Midfielders: Mario Suarez, Sergio Busquets, Mikel San Jose, Koke, Andres Iniesta, Santi Cazorla, David Silva, Vitolo, Isco
Forwards: Cesc Fabregas, Alvaro Morata, Pedro, Diego Costa