Southampton defender Ryan Bertrand was not involved in England's training session at St George's Park ahead of the friendly against Germany.
The left-back is being assessed by the national team's medical department after picking up a knock in the weekend game against Liverpool, while Arsenal striker Danny Welbeck also sat out the session as he is on a different programme, as agreed with his club.
Raheem Sterling and Joe Hart have already withdrawn from the squad after sustaining muscle injuries while playing for Manchester City over the weekend.
Manager Roy Hodgson presented Stoke City goalkeeper Jack Butland with his England under-21 player of the year award prior to the session on Tuesday afternoon.
Uncapped Leicester City midfielder Danny Drinkwater trained with the national squad for the first time.
England fly to Berlin on Friday for their game against Germany on Saturday March 26 before facing the Netherlands on Tuesday March 29.