Barcelona central defender Samuel Umtiti misses out (knee), while midfielder duo Sergio Busquets and Arturo Vidal are both suspended for the Catalans.
Forward Ousmane Dembele trained on Wednesday as he continues his recovery from a long-term hamstring injury, although the game probably comes too soon for the Frenchman.
Image:Antoine Griezmann is congratulated after scoring against Napoli in the first leg
However, Antoine Griezmann, Clement Lenglet and Frenkie de Jong are all available after recent injuries and Ivan Rakitic may start in midfield.
Meanwhile, Napoli are set to be without centre-backs Kostas Manolas (rib) and Nikola Maksimovic (knee) with injury, although the visitors expect key striker Lorenzo Insigne to feature after recovering from the thigh injury he sustained against Lazio last weekend.
Follow Barcelona vs Napoli on Sky Sports' digital platforms with a dedicated live blog from 6.45pm on Saturday; kick-off 8pm.
Image:Dries Mertens celebrates his first-leg goal against Barcelona
Opta facts
Barcelona are set to host Napoli for the first time in a competitive match. The last team to win their first away Champions League game at Camp Nou was Rubin Kazan in October 2009 (2-1).
Napoli's two previous away Champions League knockout matches have ended in defeat, losing 1-4 to Chelsea in March 2012 and 1-3 to Real Madrid in February 2017.
Barcelona have progressed from each of their last 19 Champions League knockout ties after avoiding defeat in the first leg away from home, only failing to progress once, going out to Juventus in the 2002-03 quarter final (1-1 first leg, 1-2 second leg).
Napoli have won just one of their last 10 away Champions League games (D4 L5), winning 3-2 at Red Bull Salzburg in October 2019. However, they are unbeaten in all three away games this season (W1 D2).
Barcelona's run of 35 home Champions League games without defeat (W31 D4) is the second-longest in the history of the European Cup/Champions League, behind Bayern Munich's 43-game run between 1969 and 1991.
Two last-16 ties remaining
Bayern Munich vs Chelsea (3-0)
Barcelona vs Napoli (1-1)
Quarter-final draw in full
Manchester City vs Lyon
RB Leipzig vs Atletico Madrid
Napoli or Barcelona vs Chelsea or Bayern
Atalanta vs PSG
Semi-final draw
Manchester City or Lyon vs Napoli/Barcelona or Chelsea/Bayern Munich
RB Leipzig or Atletico Madrid vs Atalanta or Paris Saint-Germain
Champions League knockout stage - key dates
Champions League last-16 second legs: August 7-8
Champions League quarter-finals: August 12-15 (Lisbon)
Champions League semi-finals: August 18-19 (Lisbon)
Champions League final: August 23 (Estadio da Luz, Lisbon)
