Bayern Munich midfielder Thiago Alcantara will be out for several weeks after tearing a ligament in his right ankle.
The Spain international had to go off in the 75th minute of Tuesday's 2-1 German Cup second-round victory over amateurs SV Roedinghausen following a foul.
"Bayern will be without Thiago for several weeks," the club said in a statement after the player's medical examination.
Thiago joins injured France internationals Corentin Tolisso and Kingsley Coman on the sidelines.
Bayern, second in the Bundesliga, take on AEK Athens in the Champions League next week before facing current German league leaders Borussia Dortmund a few days later.