Tyrone Mings will still join up with England on Monday, but he will be assessed by FA medical staff ahead of the upcoming World Cup Qualifiers after suffering a broken rib.
It comes as Gareth Southgate awaits news on Dominic Calvert-Lewin, who has played in all three Premier League games for Everton this season with a broken toe, and also picked up a thigh strain in Saturday's win over Brighton.
All members of the England squad are due to report to St Georges Park by Monday lunchtime.
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While Southgate has cover at centre back with Harry Maguire, John Stones, Conor Coady and Kyle Walker, who can play in a three-man defence, he has very limited striker options if Calvert-Lewin is forced to withdraw.
Harry Kane played his first full 90 minutes since the Euros final, in Sunday's 1-0 win for Tottenham over Watford. But first-time call-up Patrick Bamford is Southgate's only other specialist striker in the squad.
The England boss named an enlarged squad of 25 for this international triple-header, as cover for potential injuries or Covid issues.
He had hoped to avoid making any late additions to the squad, but he will have a decision to make on that front, if Calvert-Lewin is not available.